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+--- Thread: Delivery of siRNA across the blood brain barrier .. Need Help !! (/thread-2606.html)
Delivery of siRNA across the blood brain barrier .. Need Help !! - anaw - 11-07-2013
Hi! my supervisor wants me to use exosomes in our model and I am trying to follow the method of Nat. Protocols El-Andaloussi et al 2012 and Alvarez-Erviti Nat Biotech 2011 but I am not getting any silencing in the brain and I have just seen these comments in you tube and in this blog talking about fraud and saying that this papers should be retracted! who should I trust? does anybody know other protocol for brain delivery of siRNAs using exosomes? I am quite desperate! any advice would be greatly appreciated!
http://www.youtube.com/watch?v=Ry9kL-R-7E8
http://metamodern.com/2011/03/22/across-the-blood-brain-barrier-with-exosomes/
Cheers
Ana
RE: Delivery of siRNA across the blood brain barrier .. Need Help !! - markcar - 02-01-2014
(11-07-2013, 02:02 AM)anaw Wrote: Hi! my supervisor wants me to use exosomes in our model and I am trying to follow the method of Nat. Protocols El-Andaloussi et al 2012 and Alvarez-Erviti Nat Biotech 2011 but I am not getting any silencing in the brain and I have just seen these comments in you tube and in this blog talking about fraud and saying that this papers should be retracted! who should I trust? does anybody know other protocol for brain delivery of siRNAs using exosomes? I am quite desperate! any advice would be greatly appreciated!Hi Ana,
http://www.youtube.com/watch?v=Ry9kL-R-7E8
http://metamodern.com/2011/03/22/across-the-blood-brain-barrier-with-exosomes/
Cheers
Ana
I would not waste the money of your grant trying it, I do not know if these papers are the result of misconduct but we tried this many times some months ago and it did not work.
Regards
Mark