Help with nitro-cellulose membrane robustness - Printable Version +- Biotechnology Forums (https://www.biotechnologyforums.com) +-- Forum: Biotechnology Discussion (https://www.biotechnologyforums.com/forum-1.html) +--- Forum: Biotechnology Products (https://www.biotechnologyforums.com/forum-7.html) +--- Thread: Help with nitro-cellulose membrane robustness (/thread-6806.html) |
Help with nitro-cellulose membrane robustness - niklas - 02-04-2015 Hi all, I'm currently developing a serological dipstick test and I have difficulties in generating robustness in my results. I'm using antibody-HRP as my detection with 4CN as a precipitate but I would like to create a SOP to increase the robustness for my assay. But when i replicate my tests I get quite high variation in the intensities in my spots. Would changing into DAB generate more reproducible results? Is it simply impossible to get a good robustness when using an enzyme reaction to visualise primary antibodies? all the best |