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+--- Thread: Biological Characterization (/thread-7152.html)
Biological Characterization - boldfacebutton7 - 10-17-2015
I am working on a project and that requires various biological characterization of the specimen. I am a mechanical engineer so most of the things are just bouncing of my head. Can anyone please explain the following two procedures
1. Cytotoxicity Test
2. Hemolysis Test
RE: Biological Characterization - walkingfish - 11-15-2015
Are trying to build a machine that does tests? I don't know what you are trying to do. If you are wondering about testing cytotoxicity of a chemical, etc., there are all sorts of ways. Toxins might act differently within an organism tha without (in vivo vs in vitro). A simple in vitro test is to have cells incubated in wells, expose them to chemical levels at various concentrations, and see if they live or not. As for hemolysis, I don't know what sort of perturbation might cause it in your case (mechanical disruption? toxin? other?). A general way to address this is to have RGBs alive in wells, expose them to the potentially offending factor, and then use a cell counter slide and a microscope to see how many are alive after the treatment relative to before the treatment.