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vector DNA - priyansha sinha - 11-10-2016

Respected sir,

I have a question regarding vector DNA change . when some foreign gene (like gene for pest resistance) is inserted into a vector say agrobacterium tumefaciens  so as to transfer this gene into plants , doesn't it harm the mechanism of agrobacterium? Doesn't it make agrobacterium resistant ?
kindly assist me giving answer of this question.

Thank you


RE: vector DNA - priyansha sinha - 12-28-2016

(11-10-2016, 04:31 AM)priyansha sinha Wrote: Respected sir,

I have a question regarding vector DNA change . when some foreign gene (like gene for pest resistance) is inserted into a vector say agrobacterium tumefaciens  so as to transfer this gene into plants , doesn't it harm the mechanism of agrobacterium? Doesn't it make agrobacterium resistant ?
kindly assist me giving answer of this question.

Thank you

please answer this question if anyone knows it correctly.


RE: vector DNA - issral khan - 12-28-2016

when we want to insert a new gene in host....ist of all the vector. having gene of intrest is inactive. and tha molecules. which. are able to gene regulations. and exprassion for gene of intrest are not present. in agrobaterium....these are olny preset in plants....so that the gene of interest. never shows it funcation in bacteria....


RE: vector DNA - priyansha sinha - 12-28-2016

(12-28-2016, 08:49 PM)issral khan Wrote: when we want to insert a new gene in host....ist of all the vector. having gene of intrest is inactive. and tha molecules. which. are able to gene regulations. and exprassion for gene of intrest are not present. in agrobaterium....these are olny preset in plants....so that the gene of interest. never shows it funcation in bacteria....

so you mean vectors (here agrobacterium) are deliberately made inactive??