Why protiens are precipitated at their isoelectric point? - Printable Version +- Biotechnology Forums (https://www.biotechnologyforums.com) +-- Forum: Information (https://www.biotechnologyforums.com/forum-16.html) +--- Forum: Questions and Answers (https://www.biotechnologyforums.com/forum-37.html) +--- Thread: Why protiens are precipitated at their isoelectric point? (/thread-8205.html) |
Why protiens are precipitated at their isoelectric point? - Fazlil - 09-29-2017 Why protiens are precipitated at their isoelectric point? RE: Why protiens are precipitated at their isoelectric point? - SunilNagpal - 09-29-2017 (09-29-2017, 07:22 PM)Fazlil Wrote: Why protiens are precipitated at their isoelectric point? At isoelectric point there is no net charge on a protein. At any other pH there remains a net electric charge on a protein which enables it to interact with water (or other related solvents i.e polar solvents). So, if a protein lacks a charge, it will not interact with the solvent (assuming it is polar); which in turn will lead to over powering of protein-protein interactions as compared to protein-water (or polar solvent) interactions; thereby leading to precipitation. RE: Why protiens are precipitated at their isoelectric point? - vishu272 - 09-29-2017 There must be defined nature of solvent , as water is a polar solvent and in above answer it should be mentioned other related solvents as 'polar solvents'. In non-polar solvents protein may not precipitate at their isoelectric ph RE: Why protiens are precipitated at their isoelectric point? - ForumsEditor - 09-30-2017 (09-29-2017, 08:55 PM)vishu272 Wrote: There must be defined nature of solvent , as water is a polar solvent and in above answer it should be mentioned other related solvents as 'polar solvents'. In non-polar solvents protein may not precipitate at their isoelectric ph Completely agree!..editing it to clearly put related (to water) solvents as Polar solvents... (@SunilNagpal, I just put an extra information regarding "Polar solvents" as the exact term for your citation of solvents related to Water) |