10-08-2012, 11:26 PM
Hello forum members,
We are looking to bind single cell genomic DNA to microbeads and perform a whole genome amplification on the beads. We want to perform a non PCR ampificiation.
The idea is to bind the DNA to the microbeads using the streptadivin - biotin system. We need the DNA bound to microbeads while leaving the DNA available for amplification, thus we want to bind the fosfate backbone with DNA binding proteins.
I have a few questions:
1) Do you think it is feasable to perform whole genome amplfication on microbeads?
2) Can annyone suggest a DNA binding protein that we could biotinylate to produce a probe that leaves the bound DNA available to amplification?
3) Do you have a different suggestion as to how we could bind DNA to microbeads, and subsequently perform a DNA amplification?
Greetings
We are looking to bind single cell genomic DNA to microbeads and perform a whole genome amplification on the beads. We want to perform a non PCR ampificiation.
The idea is to bind the DNA to the microbeads using the streptadivin - biotin system. We need the DNA bound to microbeads while leaving the DNA available for amplification, thus we want to bind the fosfate backbone with DNA binding proteins.
I have a few questions:
1) Do you think it is feasable to perform whole genome amplfication on microbeads?
2) Can annyone suggest a DNA binding protein that we could biotinylate to produce a probe that leaves the bound DNA available to amplification?
3) Do you have a different suggestion as to how we could bind DNA to microbeads, and subsequently perform a DNA amplification?
Greetings
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