[Ikariotis]

I am trying to express human protein in E.coli, but some of them are not getting expressed at all or in a very small amount. I realize that there can be many parameters that affect protein expression at cellular level (like codon usage for example) but I am more interested in parameters in the physiology of cultivation like media and the cell lysis. Today we are using Tryptic soy broth + yeast extract and for cell lysis, lysis buffer. Can a change this two parameters for better protein expression and where can i find resources for this?