[brijnbhatt]

Dear member,

I saw your question just now. Well, you can clone multiple genes in same vector. As it is Eukaryota system, it is important to have following things for expression.

1- Suitable promoter for your system (Plant specific promoters)
2- Ribosome binding sites (for initiation of translation)
3- Transcription termination (Termination of transcription)

If you want to added multiple gene in same vector and you have to duplicate theses expression elements then vector size will be very big and may cause difficulties for cloning and further use in expression.

There are several IRES (Internal Ribosome entry sites) known for plant system and you can add any of these sites between your two genes. As there will be translation termination codon for your both the genes they will be transcribed with same promoter and transcription terminator but two different proteins will be synthesized.

Please read these two articles, it will help you

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC157974/
http://www.ncbi.nlm.nih.gov/pubmed/12848831

For more advancement in your system, you may use tissue specific promoters to your vector.

Here below i have given a link for the list of vectors which you can use for your system.

http://www.arabidopsis.org/abrc/catalog/vector_1.html

Hope you will be able to find solution from my answer, if you have any further question, please write it here. I will try
to attend that.

good luck

Brijesh