We used cre-loxp technology to knockout one gene in mouse brain. We have confirmed the targeted gene was knockout at first two weeks after this gene deletion at protein and mRNA level. But I can not confirm it after 3 and 9 month of gene deletion.
Cre induced recombination is permanent and it cannot reverse later. But now how to explain our experiment? What are the possible reasons?
Cre induced recombination is permanent and it cannot reverse later. But now how to explain our experiment? What are the possible reasons?
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