[CHANDA7]

#Chanda7

Apologize. I used the Set A question paper. Image did not load.

I think the picture below summarizes. If it had a correct orientation(1st plasmid) - BamHI + HindIII  == 0.5 + 4.5
                                                                                                                              PstI    + HindIII   == 1.5 + 3.5
Answer is C. It will be reversed(A) for incorrect orientation. 

Thanks bro..... I didn't think it's so easy