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restriction mapping problems
#1
Hi all! Any ideas how to solve these problems? Any help would be greatly appreciated! ThanksBig Grin


Problem 1:
A linear piece of DNA is cut with restriction enzymes HindIII, BamHI, and EcoRI in single, double and triple digests. The resultant restriction fragments are separated by electrophoresis in an agarose gel. The sizes (in kb) of the fragments are indicated below. Construct a well-labeled restriction map from this data. If two fragments are the same size, they are listed once.
BamHI HindIII EcoRI BamHI+ BamHI+ HindIII+ BamHI+
HindIII EcoRI EcoRI EcoRI+
HindIII
3kb 5kb 1kb 2kb 1kb 1kb 1kb
7kb 9kb 3kb 3kb 4kb 2kb
5kb 6kb 5kb 3kb
4kb
RESTRICTION MAP: ?


Problem 2.
A plasmid sample was digested with three enzymes (Pst1, BglII, and EcoRI) in single, double and triple digests. The resultant restriction fragments are separated by electrophoresis in an agarose gel. The sizes (in kb) of the fragments are indicated below. Construct a well-labeled restriction map from this data. If two fragments are the same size, they are listed once.
PstI BglII EcoRI PstI+ Pst+ BglII+ Pst+
BglII EcoRI EcoRI Bg1II+
EcoRI
4.4kb 16.9kb 6.1kb 2.1kb 2.6kb 4.9kb 2.1kb
12.5kb 10.8kb 2.3kb 3.8kb 5.9kb 2.3kb
12.5kb 4.4kb 6.1kb 2.6kb
6.1kb 3.8kb
6.1kb
RESTRICTION MAP:




Problem 3. A plasmid sample was digested with three enzymes (NotI, EcoRI, and XhoI) in single, double, and triple digests. The resultant restriction fragments are separated by electrophoresis in an agarose gel. The sizes (in kb) of the fragments are indicated below. Construct a well-labeled restriction map from this data. If two fragments are the same size, they are listed once.
NotI EcoRI XhoI NotI+ NotI+ XhoI+ NotI+
EcoRI XhoI EcoRI EcoRI+
XhoI
3.8kb 21.9kb 10.5kb 3.8kb 3.8kb 4.1kb 3.8kb
19.4kb 21.6kb 11.4kb 7.9kb 5.1kb 6.4kb 4.1kb
20.3kb 21.6kb 10.1kb 10.5kb 11.4kb 5.1kb 10.2kb 16.5kb 15.2kb 6.4kb
11.5kb 10.1kb
RESTRICTION MAP:?
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