[ArtursShuleiko]

Hello, my name is Arthur. 

I am willing to explore perfusion bioreactor cultivation specifics. Almost all of the information I have gathered over the past 2 months are articles, in which researchers use perfusion bioreactor setups for cultivating mammalian cells. I am willing to perform a similar experiment using TFF, but there is a problem, considering that all mammalia cells seem to be very sensitive to contamination. I won`t be able to perform the experiment on mammalian type cultures, due to the lack of required sterility of the lab in which I work.
Is it possible to perform cultivation processes using perfusion on microbial or yeast cultures? I have performed numerous fermentation processes on Escherichia coli and Saccharomyces cerevisiae in a fed-batch mode, but haven`t found any information about weather I can perform perfusion cultivations on these cultures or some other cultures that do not require a very high level of sterility.

If someone has some experience on this topic or some ideas, please help me with this problem. Also, if someone has some advise about performing perfusion cultivations I would gladly read it. Thank you for your time.))

[SunilNagpal]

Hello, my name is Arthur. 

I am willing to explore perfusion bioreactor cultivation specifics. Almost all of the information I have gathered over the past 2 months are articles, in which researchers use perfusion bioreactor setups for cultivating mammalian cells. I am willing to perform a similar experiment using TFF, but there is a problem, considering that all mammalia cells seem to be very sensitive to contamination. I won`t be able to perform the experiment on mammalian type cultures, due to the lack of required sterility of the lab in which I work.
Is it possible to perform cultivation processes using perfusion on microbial or yeast cultures? I have performed numerous fermentation processes on Escherichia coli and Saccharomyces cerevisiae in a fed-batch mode, but haven`t found any information about weather I can perform perfusion cultivations on these cultures or some other cultures that do not require a very high level of sterility.

If someone has some experience on this topic or some ideas, please help me with this problem. Also, if someone has some advise about performing perfusion cultivations I would gladly read it. Thank you for your time.))

Hi Arthur,

Yes you can use Perfusion for Yeast. It has rather been successfully tried by many research groups. Coussa et al., 2012 used microencapsulated yeast for urea removal from perfused plasma stream. Douglas et al., 1985 also used a 'close to perfusion' set up for Bioetahnol production. Goes without saying that perfusion is suited for biosynthesis of 'products (secondary often)' and are not at all useful for biomass cultivation (given their 'continous mode where a steady state is needed'). 
Why do you want to use a perfusion set-up for yeast/bacteria? It would definitely be useful for increasing productivity of some target bioproduct, but if it is for routine cultivation of the cells then I would say that the conventional bioreactors (which you are an expert of I am sure) would be most suitable.

Hope it helps.
Let me know if I can be of any/further help.

Best wishes

[ArtursShuleiko]

Thank you Sunil!

I want to perform an experiment using perfusion mode to determine weather or not our computer applications for modelling and monitoring culture behavior during fermentation processes will work properly, and also to see how our apparatus will handle it. But to be sure before performing an experiment we want to make it clear, from the theoretical point of view, that there will be no problems with the filter fouling or something like that.

Thank you for your help. Maybe, if you can give us some more advice on this topic, It would be helpful.))