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In cell culture, can treated surface survive autoclaving?
#1
This is post no. 1 under the main topic.
If I treat a surface for cell adhesion with fibronectin, collagen, etc., can I autoclave it and expect it to function in allowing the attachment of mammalian cells?  Since denatured collagen is used to promote adhesion, it seems like it might be feasible, but I don't know.  If this is a less than optimal idea, does anyone know of any alternatives?  If anyone has any insight, I'd appreciate it.  I'll thank you in advance.
 
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#2
This is post no. 2 under the main topic.
(11-09-2015, 02:45 PM)walkingfish Wrote: If I treat a surface for cell adhesion with fibronectin, collagen, etc., can I autoclave it and expect it to function in allowing the attachment of mammalian cells?  Since denatured collagen is used to promote adhesion, it seems like it might be feasible, but I don't know.  If this is a less than optimal idea, does anyone know of any alternatives?  If anyone has any insight, I'd appreciate it.  I'll thank you in advance.

Heating does lead to gelatinization of collagen, but you can't use too concentrated gelatin for adhesion. So, I am not sure if that would be an optimal way out. Ideally you should be using filter sterilized collagen solution for treating your (autoclaved) surface under the hood. That is more of a standard procedure.

Hope it helps
 
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#3
This is post no. 3 under the main topic.
Thank you very much.
 
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