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GATE BT-2017 Answer Key | Solutions Discussion
#13
Q. ELISA is used to
Ans. To determine antibody level
#14
(02-05-2017, 10:26 PM)RONIT SHARMA Wrote: Q. Enzyme conc. is doubled in reaction following MM kinetics. Assume [So]>>Km. Find correct statement.
Options: I don't remember but one of ans was: There is no effect on Km and Vm and I market it.

In my understanding of things, Km shouldn't change but Vm will change if enzyme concentration is doubled.

Edit:
Assuming there were 10 enzymes each with 1 active site, and with an ability to convert 1 substrate per minute, then if there is a very high concentration of substrates (say 1000), the max rate of conversion shall remain 10 substrates per minute. Now no matter how much substrate we put further, the Vmax is not going to change. But same is not true for the amount of enzyme. Increasing the enzyme concentration will lead to increased active sites and greater Vmax. But since Km is the substrate concentration at which rate is Vmax/2 for an enzymatic reaction, so that stands as a constant.
Sunil Nagpal
MS(Research) Scholar, IIT Delhi (Alumnus)
Advisor for the Biotech Students portal (BiotechStudents.com)
Computational Researcher in BioSciences at a leading MNC


Suggested Reads:
Top Biotech Companies | Top places to work
Indian Biotech Companies and Job Openings
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Biotechnology Competitive Exams in India
#15
Can anyone tell me how many seats are there for Biotech students in IIT's and NIT's
#16
(02-05-2017, 11:35 PM)RONIT SHARMA Wrote: Can anyone tell me how many seats are there for Biotech students in IIT's and NIT's

http://www.biotechnologyforums.com/thread-7777.html
Sunil Nagpal
MS(Research) Scholar, IIT Delhi (Alumnus)
Advisor for the Biotech Students portal (BiotechStudents.com)
Computational Researcher in BioSciences at a leading MNC


Suggested Reads:
Top Biotech Companies | Top places to work
Indian Biotech Companies and Job Openings
Aiming a PhD in Top Grad School? | These are the Important Points to Consider
Careers in Biotechnology | A list of various Options
Biotechnology Competitive Exams in India
  

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