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+--- Thread: fusion gene (/thread-7438.html)
fusion gene - priyansha sinha - 10-18-2016
Respected sir,
I am a B tech Biotech student . while I was trying to learn the method to create fusion gene for a transgenic plant , I was unable to understand some points. The method was given like this..."A primer was designed to introduce a BamH1 site and to remove the translational stop codon at 3' end of cry1B gene sequence.such a step preserve the protease processing site in cry1B sequence. The cry1B gene was amplified and restricted with Nco1 and BamH1 and cloned in pBluescript KS+......"
So, I am unable to get this (i) preservation of protease processing site , and (ii) pBluescript KS+ .
kindly help me regarding these concepts and also, elaborate me the process of gene fusion.
Thank you.