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+--- Thread: Question about electrophoresis of GAPDH-fragment (/thread-8401.html)
Question about electrophoresis of GAPDH-fragment - Shinu - 06-07-2018
Hi everyone!
I have a question about an electrophoresis of the GAPDH-fragment.
We made a master mix with the following:
- Puffer Y
- dNTPs
- Primer forward & reverse
- Taq Polymerase
- DNA template
- PCR water
Now I got the 1 band (x) which I don't know what it is. I thought of mutations probably or the remaining DNA-template that didn't get cut as it should. (Picture is attached)
Does anyone know what it might be?
Thanks in advance!!=)