[SunilNagpal]

Nice!
Thankyou.

I have come up with another question though

Each column might have its own best suited solvents and their gradient concentrations, So was looking for C18, Biphenyl and C4 solvents for proteomics applications!
If anyone could help on this.

Thankyou!

Hi Kanny,

The answer is two way:
1) Columns are indeed suited to a particular set of solvents
2) One must take care of the chemical nature of the analyte to avoid its reaction/deterioration/dissolution in/with solvent(s).

• C18 is a highly non-polar stationary phase, so using a polar solvent is the most recommended one (often [Water: HPLC grade water with 0.1% acid] with different proportions of [metahnol/acetonitrile/propanol: again HPLC grade] are sufficient).
  
• Acetonitrile and Acetic acid (low conc) can be used with C4. People recommend/suggest THF too (but that can be nasty for your column. You may use higher proportions of THF/Acetic acid in C18s or C8s too for the cases where protein/peptide is resisting elution.
  
• A mix of Methanol (~70%) and water (~30%) is often considered ideal for Phenyl packings.
  

Cleaning Practice for Reverse Phase Columns (like C18, C8, C4, C1, C30, CN or Phenyl packings)

Regeneration of RP packings RP- packings like C18, C8, C4, C1, C30, CN or Phenyl  stationary phases should be cleaned through following procedure (source: nestgrp): 

• Flush the column with 20 column volumes Water
• Flush the column with 20 column volumes Acetonitrile
• Flush the column with 5 column volumes Isopropanol
• Flush the column with 20 column volumes Heptane
• Flush the column with 5 column volumes Isopropanol
• Flush the column with 20 column volumes Acetonitrile