[CHANDA7]

Answer this question please.....
Two experiments were performed. In the first one, okazaki fragments were prepared from a replicating cell of E. Coli grown in the presence of 32P. In the other, the two strands of E. Coli chromosome were separated into a H strand and L strand, immobilized onto a nitrocellulose membrane and hybridized with the okazaki fragments prepared in the first experiment. Which one of the following options correctly describes the observation?

1. Okazaki fragments will hybridize to only Have strand

2. Okazaki fragments will hybridize to only L strand

3. Okazaki fragments will hybridize with both H and L strands

4. Because the H and L strands have been prepared from different cultures of E. Coli, the okazaki fragments will hybridize to neither