[SunilNagpal]

Hi Kanny,

I don't have any data per se, but during my own research at IIT Delhi, I also used Agilent system. It has been a convention there to use Agilent columns.

My work was on bio-pesticide production, reverse phase was thus very much good to go for.

C18 is known to have highest hydrophobicity and a very broad spectrum of interaction (with a variety of compounds). C8 would just help in lower retention times (and faster elution). As C18 works with most of the organic compounds and is both cheap and very stable, I would suggest C18 for any kind of 'organic work'.

At the end of the day, Choice of stationary phases is very much dependent upon the analyte at hand. And choice of molecular wight range/pore size for your column would thus depend upon the type(s) of molecule(s) you are going to work upon and the pressure you are going to expose the column to.

The manual for columns usually helps sufficiently to address most of your needs..