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GATE BT-2017 Answer Key | Solutions Discussion
I think this one is meant for challenge, can anyone justify this!!

Question Number :10 G-ve bacteria with saffraanin, G-ve have outer membrane with LPS,
Given answer :1 , but answer according to me is 2 as staining depends on peptidoglycan layer . and its thickness

other user:
Even I think it's option B
G-ve loses crystal violet because CV-iodine complex easily washes out during alcohol wash. Whereas, in G+ve, due to thick peptidoglycan layer, CV-iodine complex cannot escape.
After CV-IODINE complex has washed out of G-ve cell, its now colourless and can take up any dye. So it gets stained with saffranin.
I don't think LPS ha any role in trapping saffranin.
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Messages In This Thread
A GATE BT Marks Survey Page - by reviewer21 - 02-28-2017, 05:31 AM
RE: A GATE BT Marks Survey Page - by SunilNagpal - 03-01-2017, 02:09 AM
RE: A GATE BT Marks Survey Page - by CHANDA7 - 02-28-2017, 04:37 PM
RE: GATE BT-2017 Answer Key | Solutions Discussion - by kashyap.aluru - 03-02-2017, 02:07 AM
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