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CSIR JRF/ UGC NET Discussions
#1
Dear Members,


This is a dedicated thread for discussions pertaining to CSIR JRF/ UGC NET. Feel free to post queries, tips and suggestions here.

------------------------------------------------------------------------

Starting with the query of forum member: CHANDA7:

This is a question from CSIR NET 2016 December..... Can anyone help me with the question number 142?

   
Sunil Nagpal
MS(Research) Scholar, IIT Delhi (Alumnus)
Advisor for the Biotech Students portal (BiotechStudents.com)
Computational Researcher in BioSciences at a leading MNC


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[+] 1 user Likes SunilNagpal's post
#2
(03-06-2017, 07:30 PM)SunilNagpal Wrote: Dear Members,


This is a dedicated thread for discussions pertaining to CSIR JRF/ UGC NET. Feel free to post queries, tips and suggestions here.

------------------------------------------------------------------------

Starting with the query of forum member: CHANDA7:

This is a question from CSIR NET 2016 December..... Can anyone help me with the question number 142?

#CHANDA7
Best possible answer is 2. Protein is in insoluble/pellet because its getting denatured at that particular temperature. Lowering temperature will prevent this and solublize the protein
#3
#Chanda7

Apologize. I used the Set A question paper. Image did not load.

I think the picture below summarizes. If it had a correct orientation(1st plasmid) - BamHI + HindIII  == 0.5 + 4.5
                                                                                                                              PstI    + HindIII   == 1.5 + 3.5
Answer is C. It will be reversed(A) for incorrect orientation. 
[Image: YHkPp4zAGs0uWplzS0MqItY_vDLbFR6571nDhsC1...60-h488-no]
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#4
(03-06-2017, 10:39 PM)travellingsalesman Wrote: #Chanda7

Apologize. I used the Set A question paper. Image did not load.

I think the picture below summarizes. If it had a correct orientation(1st plasmid) - BamHI + HindIII  == 0.5 + 4.5
                                                                                                                              PstI    + HindIII   == 1.5 + 3.5
Answer is C. It will be reversed(A) for incorrect orientation. 
[Image: YHkPp4zAGs0uWplzS0MqItY_vDLbFR6571nDhsC1...60-h488-no]
Thanks bro..... I didn't think it's so easy
#5
Can anyone help me with this question?
( For search: IPTG, T7 RNA polymerase, lac operator, cell free supernatant)



[Image: 6204c4dc0fcca1e96f8c34abadf03fa5.jpg]
#6
    can anybody help me with these two questions?
#7
(03-08-2017, 03:56 PM)CHANDA7 Wrote: Can anyone help me with this question?
( For search: IPTG, T7 RNA polymerase, lac operator, cell free supernatant)



[Image: 6204c4dc0fcca1e96f8c34abadf03fa5.jpg]


I answered this question before(accidently)
Best possible answer is 2. Protein is in insoluble/pellet because its getting denatured at that particular temperature. Lowering temperature will prevent this and solublize the protein
[+] 1 user Likes travellingsalesman's post
#8
(03-28-2017, 09:41 PM)travellingsalesman Wrote:
(03-08-2017, 03:56 PM)CHANDA7 Wrote: Can anyone help me with this question?
( For search: IPTG, T7 RNA polymerase, lac operator, cell free supernatant)



[Image: 6204c4dc0fcca1e96f8c34abadf03fa5.jpg]


I answered this question before(accidently)
Best possible answer is 2. Protein is in insoluble/pellet because its getting denatured at that particular temperature. Lowering temperature will prevent this and solublize the protein
Thanks bro..... Can u explain what could be the expected changes as of other options?
#9
    can anybody answer this with justification???
Please help me.....

Key for search: Circular Dichroism, psychrophile, thermophile, protein....
#10
May be the answer is : d.. bcz of salt bridge made of by the hydrogen bond and we knw H- bonding highly stable...for that reason last one is the answer...what is the answer???
[+] 1 user Likes supriyo banerjee's post
  


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