How to increase the frequency of mutation by using GeneMorph II Random Mutagenesis Kits (Error prone PCR/directed evolution)? 
Is there any conventional methods to introduce mutation in lipase gene to make it thermostable? 
Any effective protocols for gene cloning and transformation after Error prone PCR?

How to increase the frequency of mutation by using GeneMorph II Random Mutagenesis Kits (Error prone PCR/directed evolution)? 
Is there any conventional methods to introduce mutation in lipase gene to make it thermostable? 
Any effective protocols for gene cloning and transformation after Error prone PCR?

Hi Sunil Sir,

First of all, my heartiest thanks to you for providing such accurate guidance in this forum. It fills us with positivity in terms of our future carrier. I completed my B.Sc (hons.)in Microbiology in Scottish Church College under Calcutta University. I am planning to pursue an M.Sc in either microbiology or biotechnology .I gave JAM this year but couldn't rank well due to lack of preparation (AIR 663). I also sat for JNU CEEB and hoping for better result. I have other exams lined up  for admission in Pondicherry Univ, BHU, VIT, Pune University and few others in Calcutta (Jadavpur, Calcutta university, Bose institute, St.Xaviers). For future, I want to work in a Biotech Company but my larger goal is teaching which i want to continue after working for few years. Now ,my first doubt is what should be my first preference for preparation-NET or GATE? If I opt for GATE, then how many more years will it take to complete Ph.D after M.Tech? And another doubt which I have is whether studying in Calcutta will affect my future result (as outside, I heard, there are better scope)?. I would prefer studying here because I can pay better attention to the competitive exams as well. What would you suggest?

One of the principle complaints of the anti-GMO crowd is that genetic engineering lines the pockets of mega-corporations rather than individual consumers. Well, what about if everyone was able to own shares in the companies working with this technology? Then we could turn the general public into evangelists for the technology and enthusiastic consumers of the resulting products.

That's the idea behind my company opening up it's seed round to the general public.

I'd love to know what this community thinks of this idea. Can it work?

Has anyone ever tried to produce MAb with a peptide (20 a.a sequence) with FCA adjuvant alone, without any carrier protein. Does it work, If so could you direct me to a paper?

the other group tried KLH conjugate. I'am trying without carrier protein with adjuvant, I don't see nice titer values. Is this even a feasible approach. 


people had a problem with carrier that antibodies were raised against the carrier and very low against desired epitope. Is there any paper that worked out peptide without carrier. I see in every book they mention associating with carrier protein .

There are four NIT which are offering M.Tech Biotechnology:
1. MNNIT ALLAHABAD
2. Maulana Azad National Institute of Technology Bhopal 
3. National Institute of Technology Durgapur 
4. National Institute of Technology, Rourkela 
My GATE score is 573. Plz help me which NIT should I preferred first?

I am a final year M.Sc. student of Calcutta university pursuing my masters in Biotechnology. I have qualified for GATE this year in Biotech paper with an AIR of 252 and GATE score of 550. I had made up my mind to pursue M.Tech after M.Sc. ; but sadly my rank was not that great in GATE 2016. I had sorted out and wish to apply in any of the three courses in different IITs and NITs which are Environmental Engineering in IIT Delhi, Kanpur and NIT Surathkal, Bioprocess engineering in IIT Roorkee and Industrial Biotech in NIT Surathkal. Sadly, I did not meet the gate cutoff criteria in IITD and IITK. So now my choice is between bioprocess engg in IITR and Industrial Biotech/ Environmental engg in NIT Surathkal.

My main motive behind this decision was to obtain high class teaching and interactive sessions in the above stated top class institutes, excel in my subject and opt for a proper placement.

I just wanted to know which amongst these subjects I have chosen, is the one with better future prospects and whether these institutes provide good placements to eligible students.


Regards,
Ronit Bose

Hello everyone...
can anyone help me regarding the admissions to CSIR LABS for mtech n phd courses...i mean whats the procedure to apply...do i ve to apply to each individual lab separately or Acsir covers evry lab...m really cnfused...
infact which labs offers courses relevent to biotech...
any info will be really helpful..

Hai, my name is gopika. im a biotechnology graduate. nw im confused thinking what i will pursue for masters. im not interested in research field. so is there any other option which i can go for where im not forced to do a research work. thank you.

Sir I am in class 11 I Have opted for physics chemistry biology and maths. I am confused weather pcm is better or pcb for biotechnology. I mean which one has the better scope.





Waiting for your reply
Aaishu

Hey.

I got an AIR of 84 in GATE 2016 (BT). How and what should I prepare for written/interview at IIT-M for MS ® programme ?

Secondly, my interview dates for IIT-Bombay (M. Tech in BE) and IIT-Kanpur (M. Tech. in Biological Sciences) are clashing. Which institute shall I choose and why and preparation tips for the same (written and interview).
P.S. It would be really helpful if data related to number of intakes (seats) and any other thing (differences) for II-B and IIT-K are provided.

I'm writing an essay on human genetic engineering and wanted to add a comedic but informative part at the end of my essay so I want to address the impact it might have on sports. What kind of impact....title. Both pros and cons please.

hi guys. im mrunalini studying cancer biotech in chennai. i wanna know about summer interships in india

what are the differences between B.Sc and B.Tech in biotechnology? 

and i am interested only in either research or biotech field. therefore what are career options for me?

could i be able to get a good job in biotech field in india??


and the last one, is it possible to do M.S in biotechnology after B.Sc in biotechnology??

Can somebody share their GATE Biotechnology written test/interview experience at IIT Madras? There's not many articles on the web regarding this and so requesting anyone to help out this year's interviewees.
Thanks

Hello Sir,

Please tell me about job opportunities after doing M.Tech in Environmental Engg. especially from IITs/NITs. What about the pay scale???

Hello sir,
Can you please tell how is Regional centre for biotechnology,faridabad for Ph.D
Also should i apply to iit ropar, jodhpur..? Are they good?

Sunil sir,
Hello i want to ask a question about PhD.
Doing PhD in 3 years from Amity university mou institute ( iari)  is best or doing PhD in IITs in 5-6 years(Normaly) is best?
is there any diffrence bw degrees? or any hurdle in job i get in future if we do PhD from amity university?

I am from CS background and have got call to attend written test/interview at Biotechnology dept @IITM. I have chosen Computational Biology as a specialisation as I have worked on gene clustering as my 4th year Btech project. Can somebody help me with the pattern/type of questions asked in the written test? They have not very clearly stated what the written test content will be?
Please help .
Thanks