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+--- Thread: Protein- common enzymes - protein purification (/thread-7192.html)
Protein- common enzymes - protein purification - warmbaby - 11-18-2015
Please I am about to carry out a few enzyme assays.
First I would like to know which are the most common enzymes carried out in organic media.
Secondly, what is the best way to carry out a protein purification to remove the solvents and what is the acceptable maximum time to keep the protein at 4 degree before performing enzyme test? Or I wonder what is the common option in your lab, test before it loses activity at 4 degree or store the protein at -80 degree for long term use?
Thanks