[brijnbhatt]

Hi,

I think the antibody from different companies are different. Means some antibodies are working good from one company but from same company other antibodies are not good. I have found most of the antibodies from Abcam are good. Or you may try from Dako or sigma. Check the literature and figure pictures for same antigen. If in literature you found any good IHC figure check the reagent and find out which company antibody they have used. It will help you to find the dilution and protocol also.

Hope it will work for you.

good luck.