Contact: to feature here

Thread Rating:
  • 0 Vote(s) - 0 Average
  • 1
  • 2
  • 3
  • 4
  • 5
Help with nitro-cellulose membrane robustness
Hi all,

I'm currently developing a serological dipstick test and I have difficulties in generating robustness in my results. I'm using antibody-HRP as my detection with 4CN as a precipitate but I would like to create a SOP to increase the robustness for my assay. But when i replicate my tests I get quite high variation in the intensities in my spots. Would changing into DAB generate more reproducible results? Is it simply impossible to get a good robustness when using an enzyme reaction to visualise primary antibodies?

all the best
Like Post Reply

Possibly Related Threads…
Last Post

Users browsing this thread:
1 Guest(s)

Help with nitro-cellulose membrane robustness00