Can anyone suggest which out of the four companies Merckmillipore, Agilent, Thermo scientific and Waters is better for chromatographic columns giving a little data supporting their answer?
According to my findings, Agilent columns are the most widely used.
Also, which columns would be more preferred for proteomics application? C18,C8,biphenyl ? and their molecular weight ranges if possible!
I don't have any data per se, but during my own research at IIT Delhi, I also used Agilent system. It has been a convention there to use Agilent columns.
My work was on bio-pesticide production, reverse phase was thus very much good to go for.
C18 is known to have highest hydrophobicity and a very broad spectrum of interaction (with a variety of compounds). C8 would just help in lower retention times (and faster elution). As C18 works with most of the organic compounds and is both cheap and very stable, I would suggest C18 for any kind of 'organic work'.
At the end of the day, Choice of stationary phases is very much dependent upon the analyte at hand. And choice of molecular wight range/pore size for your column would thus depend upon the type(s) of molecule(s) you are going to work upon and the pressure you are going to expose the column to.
The manual for columns usually helps sufficiently to address most of your needs..
Pleasure is all mine Kanny!
I will do some research to look for information on Mol wt ranges for various columns.
If you get to know the same, do share it..that would be a useful one.
In general, molecular weight range in case of RP should be associated with 'pore size' of the column (larger peptides would need a wider column, while smaller peptides may be dealt with a narrow one as well). Since it's not a 'molecular sieve' chromatography, the stationary phase (C18) should be expected to have a universal range.
At the same time, it would be an interesting piece of information, as to what's the 'handling limit' of C18 stationary phase in general.