03-28-2017, 09:41 PM
(03-08-2017, 03:56 PM)CHANDA7 Wrote: Can anyone help me with this question?
( For search: IPTG, T7 RNA polymerase, lac operator, cell free supernatant)
I answered this question before(accidently)
Best possible answer is 2. Protein is in insoluble/pellet because its getting denatured at that particular temperature. Lowering temperature will prevent this and solublize the protein