[SunilNagpal]

Dear Members,


This is a dedicated thread for discussions pertaining to CSIR JRF/ UGC NET. Feel free to post queries, tips and suggestions here.

------------------------------------------------------------------------

Starting with the query of forum member: CHANDA7:

This is a question from CSIR NET 2016 December..... Can anyone help me with the question number 142?

   

[travellingsalesman]

Dear Members,


This is a dedicated thread for discussions pertaining to CSIR JRF/ UGC NET. Feel free to post queries, tips and suggestions here.

------------------------------------------------------------------------

Starting with the query of forum member: CHANDA7:

This is a question from CSIR NET 2016 December..... Can anyone help me with the question number 142?

#CHANDA7
Best possible answer is 2. Protein is in insoluble/pellet because its getting denatured at that particular temperature. Lowering temperature will prevent this and solublize the protein

[travellingsalesman]

#Chanda7

Apologize. I used the Set A question paper. Image did not load.

I think the picture below summarizes. If it had a correct orientation(1st plasmid) - BamHI + HindIII  == 0.5 + 4.5
                                                                                                                              PstI    + HindIII   == 1.5 + 3.5
Answer is C. It will be reversed(A) for incorrect orientation. 

[CHANDA7]

#Chanda7

Apologize. I used the Set A question paper. Image did not load.

I think the picture below summarizes. If it had a correct orientation(1st plasmid) - BamHI + HindIII  == 0.5 + 4.5
                                                                                                                              PstI    + HindIII   == 1.5 + 3.5
Answer is C. It will be reversed(A) for incorrect orientation. 

Thanks bro..... I didn't think it's so easy

[CHANDA7]

Can anyone help me with this question?
( For search: IPTG, T7 RNA polymerase, lac operator, cell free supernatant)

[CHANDA7]

    can anybody help me with these two questions?

[travellingsalesman]

Can anyone help me with this question?
( For search: IPTG, T7 RNA polymerase, lac operator, cell free supernatant)

I answered this question before(accidently)
Best possible answer is 2. Protein is in insoluble/pellet because its getting denatured at that particular temperature. Lowering temperature will prevent this and solublize the protein

[CHANDA7]

Can anyone help me with this question?
( For search: IPTG, T7 RNA polymerase, lac operator, cell free supernatant)

I answered this question before(accidently)
Best possible answer is 2. Protein is in insoluble/pellet because its getting denatured at that particular temperature. Lowering temperature will prevent this and solublize the protein

Thanks bro..... Can u explain what could be the expected changes as of other options?

[CHANDA7]

    can anybody answer this with justification???
Please help me.....

Key for search: Circular Dichroism, psychrophile, thermophile, protein....

[supriyo banerjee]

May be the answer is : d.. bcz of salt bridge made of by the hydrogen bond and we knw H- bonding highly stable...for that reason last one is the answer...what is the answer???