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Standardization of qPCR gene expression data from different biological replicates
#1
Hey,
I have performed three experiments on gene expression studies of drug treated and untreated samples of lung cancer cell lines to quantify the expression profile of 11 genes with 1 reference gene using quantitative real time PCR. This was performed with three biological replicates and two technical replicates for each biological replicate. I have observed a general trend in the regulation of the genes. However, these three experiments were performed on three different plates and so I would like to know how to compile the results from all the three experiments to reach a proper conclusion.
How do I perform a statistical analysis of the data?
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Standardization of qPCR gene expression data from different biological replicates00